Processing Varian DEPT Data
Varian’s automated DEPT pulse program generates an arrayed experiment with 4 slices, acquired with phase of the last pulse equal to 45, 90, 90, 135 degrees. The macro below FTs all slices, then combines them to yield an edited stacked plot.
|After FT but before "editing", the slices are (top to bottom):|
|After "editing", the slices are|
First, import the data, FT and phase the first spectrum, to determine the phase correction.
NUTSMacro edited DEPT ; for Varian std DEPT sequence ; where slices 1 thru 4 are acquired with angle of last ; pulse = 45, 90, 90, 135 ; first, import arrayed data, FT 1st slice and phase ; final result is stacked plot with slices ; displayed, from top to bottom: ; CH3 ; CH2 ; CH ; all protonated ; macro asks for file name for imported FID array ask filea ga set sl 1 set filec dept.2d ; first, FT all slices ga bc em ft ps sc in set filea dept.2d ga set sl 1 ga ; 1st slice contains all peaks positive ; multiply it by 3 and save set am 2 al as set fileb dept.1 sb ; slice 4 + 1.3*slice 1 removes CH2 ga set sl 1 ga set am 1.3 al set sl 4 ga as ; slice 2 minus this leaves only CH3 ; but inverted set pa 180 set am -1 al set sl 2 ga as set fileb dept.4 pc sb ; slice 3 minus 2*slice 4 eliminates CH peaks, ; leaves CH2 up, CH3 down ga set sl 4 ga set am -2 al set sl 3 ga as ; now add this to inverted CH3 only set am 1 al set sl 1 set fileb dept.4 gb as set fileb dept.3 sb ; save 2 x slice 2 for CH only set filea dept.2d ga set sl 2 ga al as set fileb dept.2 sb ; finally, combine edited slices into 2D file set filea dept.1 set filec e_dept.2d ga ga sc in set filea e_dept.2d ga ss mf set ac .5 ac set spplotx 0 set spploty 2 sp end
See also: Varian 2D processing
Last updated: 4/9/04