Using the cursor and Zoom routine.
This section illustrates use of the Zoom expansion routine, and using the cursor to set the chemical shift reference and to measure chemical shifts and coupling constants.
Choose Start Zoom Operation from the View menu. Note that typing ZO on the keyboard is equivalent.
To select a region for expansion, press and hold the left mouse button, and drag across the chosen region, which is highlighted in red.
To display this expanded region, choose Zoom Region from the Display menu or type Ctrl-E.
To return to displaying the full spectrum, choose All Reals from the Display menu, or type Ctrl-F.
Specific frequency limits can be entered by choosing Set Frequency Limits from the Display menu, or by typing F. Limits can be set in points, Hz or ppm.
The right hand half of this dialog box applies to the second dimension of 2D data.
Exit the Zoom subroutine by selecting Exit Zoom from the File menu or by typing <Enter>. The currently expanded region remains on the screen.
For details, see description of the Zoom subroutine.
The cursor can be used to set a chemical shift reference. This is done from the base level of NUTS, not from within the Zoom routine.
Press and hold the left mouse button, and place the cursor on the reference peak. While holding down the mouse button, type O (Offset) on the keyboard.
A dialog box is displayed allowing the frequency at the cursor position to be entered.
As before, the section labeled Vertical Dimension applies to 2D data.
The cursor can be used to display the frequency of any point in the spectrum.
Press and hold the left mouse button to display a cross-hair cursor. The location of the cursor is displayed at the bottom of the screen as point number, Hz and ppm.
The height of the horizontal cursor is also displayed, expressed as a percentage of the tallest peak in the spectrum.
Frequency differences can be measured using the cursor in difference mode.
Press and hold the left mouse button and position the cursor on the first peak of interest. Click once with the right mouse button, without releasing the left button. This sets a marker cursor (green).
(Alternately, or for users with a single button mouse, pressing the period key on the keyboard can be used instead of clicking the right mouse button.)
While still holding down the left mouse button, move the cursor. The position of that red cursor is shown as before, but above it is displayed the frequency difference between the marker cursor and the current red cursor position.
A multiplet can be labeled with splitting values in Hz. While the difference information is displayed, typing L on the keyboard will create a text annotation with the frequency difference in Hz. This can be edited, etc, from the Notes subroutine.
Last updated: 12/8/06