Tricks with Buffers

Here is an example of how the Buffers (BU) routine can be used to manipulate spectra. 

Let's say we want to "remove" the CH₂ peak from this spectrum of ethylbenzene, replacing the peak with noise.

First select a suitable region of noise with which to replace the peak.  Use the Zoom subcommand F to select a known spectral region, in this case 5.0 - 4.5 ppm.  

Expand to this region, exit Zoom, enter the Buffers routine (BU) and transfer the selected region to a Buffer (buffer #2 here).  Enable viewing of buffers from outside the BU routine, from the View menu, so we can watch what we're doing.  Exit Buffers.

Because buffers are always aligned by chemical shift, we need to change temporarily the shift reference of our spectrum, to line up the region around the CH₂ peak with the captured noise region.  We need a 0.5ppm region in this example.  Using the cursor, set a point to the left of CH₂ to be 5.0ppm. 

Then, use Zoom subcommand F to display 5.0 - 4.5 ppm.  Use the Ctrl-Z command in Zoom to zero out this region.

Viewing the whole spectrum, it should look like this.  Now, enter the Buffers routine and add buffer #2 to the current spectrum, and exit.

After resetting the chemical shift reference back where it belongs, we see the final result:

This is intended as an example of how NUTS tools can be combined to accomplish a task.  Obviously, this sort of manipulation should be applied with care!

Last updated 5/6/99.